Goat anti Mouse IgG Biotin Labeled

Our secondary antibodies are isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose gels. Affinity purification helps eliminate nonspecific antibodies, resulting in increased sensitivity and specificity, with decreased background. These antibodies are further purified by passing them through a column of immobilized serum protein from other species. This additional step minimizes cross-reactivity or other species serum proteins. These secondary antibodies have broad applicability and can be used in a wide range variety of laboratory techniques, including ELISA, immunoblotting, Immunohistochemistry and Cell/ tissue staining. Antibodies are stable for 12 months at 4°C.

Product Specification Sheet

Product: Biotin Conjugated Affinity Purified Goat anti-Mouse IgG (H+L) (minimal cross-reaction to Human Proteins)

Code: A111250

Size: 1.0 MG

Antibody Concentration: 0.5mg/ml

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.5% Sodium Azide

Applications:

Immunoblotting

ELISA

Immunohistochemistry

Recommended Dilutions:

ELISA 1:5000-1:20000

Immunoblotting 1:1000-1:5000

Purity and Specificity: The antibody was isolated from antisera by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. The antibody was further purified by absorption to Human proteins coupled to agarose beads.

This product has been tested by ELISA to ensure minimal cross-reaction with Human proteins.

Storage Conditions: Store at 4 C or aliquot at –20 for long-term storage

Note: This product is for research use only, not for therapeutic or diagnostic use.

For more information, please contact us at:

Phone: 205-419-0613 Fax: 205-620-1156

Email: info@proteinbiosystems.com