Protein G Purification

Protein Biosystems offers flexible, modular and affordable custom services with multiple options designed to suit your needs. A comprehensive line of monoclonal and polyclonal antibody services are listed below. Most of our staff has over 15 years of experience producing high quality immunological reagents for the research community. We deliver quality by meeting and/or exceeding the expectations of our customers and consistently delivering our products and services to specifications on schedule. We bring innovation to our customers through new and improved antibody technologies and the application of these technologies to create new and important products. Our methods are tested, tried, and true; however, we implement new technologies that have proven to deliver superior results in a cost effective manner.

Custom Services

Service: Protein G Purification

Code: C005

Description: Protein G is a bacterial cell wall protein isolated from group G Streptococci. Like Protein A from Staphylococcus aureus, Protein G binds to most mammalian immunoglobulins primarily through their Fc regions. Native Protein G contains two immunoglobulin binding sites, as well as albumin and cell surface binding sites. In the recombinant form of Protein G, these albumin and cell surface binding sites have been eliminated to reduce nonspecific binding when purifying immunoglobulins W

We will purify up to 100 mgs of your Sera, ascites fluid or tissue culture supernate by affinity chromatography using protein G coupled to agarose gels. This will produce an IgG fraction with purity greater than 95% IgG. Table 2. Purification characteristics of IgG species using the Protein A and Protein G Source Protein A Protein G Human H H Mouse H H Rabbit H H Rat L M Goat L H Cow L H Sheep L H Horse L H Guinea Pig H L Pig H L Chicken N N Hamster M M Donkey M H Legend: H = high recovery, M = medium recovery, L = low recovery, N = no recovery

All custom services are accepted and undertaken on a "best efforts" basis only. Protein Biosystems cannot and does not make any representations or warranties, expressed or implied, including any warranty of merchantability or fitness for any particular purpose for any material produced or service provided; and, all charges are due and payable as billed regardless of the outcome of our work.

For more information, please contact us at:

Phone: 205-419-0613 Fax: 205-620-1156

Email: info@proteinbiosystems.com

 

Protein G Purification
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  • Item #: C005
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Price $472.00